Cold-adapted recombinants of influenza a virus in MDCK cells I. Development and characterization of A/Ann Arbor/6/60 × A/Alaska /6/77 recombinant viruses
Identifieur interne : 002676 ( Main/Exploration ); précédent : 002675; suivant : 002677Cold-adapted recombinants of influenza a virus in MDCK cells I. Development and characterization of A/Ann Arbor/6/60 × A/Alaska /6/77 recombinant viruses
Auteurs : Takato Odagiri [États-Unis] ; Dan C. Deborde [États-Unis] ; Hunein F. Maassab [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1982.
English descriptors
- Teeft :
- Academic press, Arbor, Attenuated, Attenuation, Biological properties, Cell cultures, Chanock, Clone, Cold adaptability, Cold adaptation, Cold variant, Cold variant virus, Constellation, Days postinfection, Deborde, Different gene constellations, Doca, Donor virus, Electrophoresis conditions, Fowl plaque virus, Gene, Gene constellation, Genetic variation, Growth curve, Growth curves, Growth rate, High virus, Influenza, Influenza virus, Influenza virus genome, Influenza viruses, Kendal, Maassab, Mdck, Mdck cells, Mutant, Negative strand viruses, Parent viruses, Permissive temperature, Phenotype, Phenotypic, Plaque, Polymerase, Polymerase genes, Polypeptide, Protein page, Recombinant, Recombinant virus, Recombinant viruses, Recombination, Rna, Rna2, Rna3, Rna5, Room temperature, Specific genes, Temperature sensitivity, Tpck trypsin, Vaccine, Variant, Viral, Virology, Virus.
Abstract
Abstract: Recombinant influenza viruses made at 25 and 33° in Madin-Darby canine kidney (MDCK) cells using the cold-adapted A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus were biologically and genetically analyzed. Eight recombinants were separated into two phenotypic groups based on cold-adapted (ca) and temperature-sensitive (ts) markers: ca and ts, ca and non-ts. The ca recombinants showed different degrees of cold adaptibility (DOCA) and different patterns of virus growth at 25°. All recombinants contained at most three genes from the cold variant A/Ann Arbor/6/60 virus (triple-gene recombinant) and most contained two or one gene from the cold variant parent (double-gene and single-gene recombinants, respectively). Further, the same three genes, RNA2, RNA3, and RNA5 (NA) were the only ca A/Ann Arbor/6/60 genes found in the various recombinants. Two clones contained all three A/Ann Arbor/6/60 genes and were both cold-adapted (ca) and temperature-sensitive (ts). All other recombinant clones were ca and non-ts, and contained RNA2 and/or RNA5 (NA). Each set of single-gene ca recombinants correlated with a different, but specific cold-adapted characteristic exhibited by their growth curves at 25°. Single-gene recombinants containing only the RNA2 of A/Ann Arbor/6/60 virus showed rapid growth early in infection and intermediate final virus yield (between the titer of virus yield for the ca A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus; while the single-gene recombinant containing only the RNA5 (NA) of A/Ann Arbor/6/60 virus showed slow growth early in infection, but a high final virus yield (equivalent to that of the ca A/Ann Arbor/6/60 parent). The double-gene recombinant containing both these genes showed both rapid growth early in infection and a high final virus yield. Thus, cold adaptation can be transferred to recombinant viruses by at least two independent genes each of which can confer the cold-adaptive property by its own pathway. The genetic basis for temperature sensitivity involves both RNA2 and RNA3, but remains ambiguous in the absence of a single-gene recombinant containing only RNA3 of the cold variant.
Url:
DOI: 10.1016/0042-6822(82)90067-8
Affiliations:
Links toward previous steps (curation, corpus...)
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Recombinant influenza viruses made at 25 and 33° in Madin-Darby canine kidney (MDCK) cells using the cold-adapted A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus were biologically and genetically analyzed. Eight recombinants were separated into two phenotypic groups based on cold-adapted (ca) and temperature-sensitive (ts) markers: ca and ts, ca and non-ts. The ca recombinants showed different degrees of cold adaptibility (DOCA) and different patterns of virus growth at 25°. All recombinants contained at most three genes from the cold variant A/Ann Arbor/6/60 virus (triple-gene recombinant) and most contained two or one gene from the cold variant parent (double-gene and single-gene recombinants, respectively). Further, the same three genes, RNA2, RNA3, and RNA5 (NA) were the only ca A/Ann Arbor/6/60 genes found in the various recombinants. Two clones contained all three A/Ann Arbor/6/60 genes and were both cold-adapted (ca) and temperature-sensitive (ts). All other recombinant clones were ca and non-ts, and contained RNA2 and/or RNA5 (NA). Each set of single-gene ca recombinants correlated with a different, but specific cold-adapted characteristic exhibited by their growth curves at 25°. Single-gene recombinants containing only the RNA2 of A/Ann Arbor/6/60 virus showed rapid growth early in infection and intermediate final virus yield (between the titer of virus yield for the ca A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus; while the single-gene recombinant containing only the RNA5 (NA) of A/Ann Arbor/6/60 virus showed slow growth early in infection, but a high final virus yield (equivalent to that of the ca A/Ann Arbor/6/60 parent). The double-gene recombinant containing both these genes showed both rapid growth early in infection and a high final virus yield. Thus, cold adaptation can be transferred to recombinant viruses by at least two independent genes each of which can confer the cold-adaptive property by its own pathway. The genetic basis for temperature sensitivity involves both RNA2 and RNA3, but remains ambiguous in the absence of a single-gene recombinant containing only RNA3 of the cold variant.</div>
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